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INDONESIA
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
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Articles 11 Documents
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Search results for , issue "Vol. 6 No. 1 (2016)" : 11 Documents clear
Balur and Improving Quality of Life Gatra Ervi Jayanti; Saraswati Subagjo
Journal of Tropical Life Science Vol. 6 No. 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.07

Abstract

Balur treatment aimed to leach free radicals overwhelmingly produced in unhealthy body with rubbed over the human body. In Balur used some amino acid and herbal such as Bawang Sabrang (Eleutherine palmifolia (L.) Merr), Kopi Balur (Mixed Coffea arabica L. with Acetosal), Coconut Water (Cocos nucifera L.), Secang (Caesalpinia sappan L.) and “Divine” smoke from cloves (Syzygium aromaticum (L.) Merr. & L. M. Perry) and Tobacco (Nicotiana tabacum L). This is an observation research of Balur application in volunteers during four years at Rumah Sehat, Malang, Indonesia. The result suggested that Balur can improve quality of life  in male volunteers i.e. 61,54% for Cancer (39 volunteers); 80% for Tumor (20 volunteers); 86,36% for Diabetes mellitus (22 volunteers); 93,33% for Heart (15 volunteers); 92,86% for Autism (28 volunteers); 72,73% for Hepatitis (11 volunteers); 100% neuron disease for (2 volunteers); 93,62% for various of disease (94 volunteers). Moreover imrovement quality of life in female volunteers i.e. 72,31% for Cancer (65 volunteers); 88,57% for Tumor (35 volunteers); 75% for Diabetes mellitus (16 volunteers); 100% for Hemorrhoid (3 volunteers); 100% for stroke (3 volunteers); 40% for Cardiomegaly (5 volunteers); 66,67% for Autism (3 volunteers); 88% for various of disease (50 volunteers) and 100% for healthy (3 volunteers). Keywords: Balur, herb in Balur process, improving quality of life
Urinary Kidney Injury Molecule-1 (KIM-1) in Early Diagnosis of Acute Kidney Injury in Pediatric Critically Ill Irma Lestari Paramastuty; Krisni Soebandiyah; Basuki B. Purnomo
Journal of Tropical Life Science Vol. 6 No. 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.06

Abstract

Acute kidney injury (AKI) often associated with a high hospital morbi-mortality rate in the intensive care unit patients. Kidney injury molecule-1 (KIM-1), has many characteristics of ideal biomarker for kidney injury. The aim of this study was to compared the temporal pattern of elevation urinary KIM-1 level following critically ill children with SCr as standart biomarker of AKI. Prospective analytic observational study was conducted during October to March 2014 in the Saiful Anwar General Hospital and Physiology Laboratory Brawijaya University. There were 13 critically ill as subjects. SCr and KIM-1 levels from all subjects were measured three times ( at admission, after 1st and 6th hour). Subjects were devided into AKI - non-AKI groups by SCr level and survivor - non survivor group at the and of the observations. Results showed that there were significantly increased levels of KIM-1 in the AKI and non-AKI and survivor-non survivor group at time point. However, we found that delta KIM-1 at time point increased significant in non AKI group and survivor group. KIM-1 at admission can diagnosed AKI in critically ill children. We conclude that urinary KIM-1 is a sensitive non-invasive biomarker to diagnosed acute kidney injury in critically ill children. Increase level of KIM-1 by time shows protective and good outcome in critically ill children.
Apigenin, an Anticancer Isolated from Macaranga gigantifolia Leaves Sofa Fajriah; Megawati Megawati; Akhmad Darmawan
Journal of Tropical Life Science Vol. 6 No. 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.02

Abstract

Apigenin, a flavonoid compound has been isolated from the ethyl acetate fraction of methanol extract of Macaranga gigantifolia leaves. Isolation and purification of apigenin conducted using chromatographic method, and chemical structure characterized based on spectroscopic data. In vitro anticancer activity test against murine leukemia P-388 cell line showed potential activity with IC50 14.13 μg/mL.Key words: apigenin, flavones, Macaranga gigantifolia, anticancer, murine leukemia P-388 cell line.
Correlation between Serum Ferritin and Cardiac Troponin I in Major Beta Thalassemia Children Muhammad Ali Shodikin; Renny Suwarniaty; Susanto Nugroho
Journal of Tropical Life Science Vol. 6 No. 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.03

Abstract

Background: Major beta thalassemia (MBT) is a hereditary disease which synthesis defect on beta chains of haemoglobin, it is cause red blood cell destruction and the symptoms of anemia. Red blood cells destruction, frequent blood transfusion and low adherence to routine use of iron chelator were cause iron accumulation in the heart, liver and endocrine organs. Accumulation of iron in the myocard can lead acute myocardial infarction. One of cardiac markers that had been used for the diagnosis of myocardial infarction was cardiac troponin I (cTnI). The aim of this research is find correlation between serum ferritin levels and cTnI in MBT children.Methods: A descriptive analytic research was conducted using a cross sectional design. The subjects were divided into 2 groups, the MBT group and the control group. In both groups perform laboratory examination to checks the serum ferritin and cTnI levels. Data were analyzed using t-test and Pearson correlation test.Result: Eleven children in the MBT group and 11 children in the control group were involved in this study. In the MBT group, the mean of serum ferritin and cTnI levels were 4292.5 μg/L and 0.22 ng/mL respectively. In the control group, the mean of serum ferritin and cTnI levels were 136.2 μg/L and 0.20 ng/mL respectively. The mean of serum ferritin levels in MBT group was higher than control, statistically significant (p= 0,0004 ). The mean of serum cTnI in MBT group was higher than control, statistically not significant (p= 0,82). In MBT group, there was a weak corellation between serum ferritin and cTnI levels (r = 0,34). Keywords: Major beta thalassemia, children, ferritin, cTnI
Genetic Diversity of Porang Populations (Amorphophallus Muelleri Blume) In Central Java and West Java Based on LEAFY Second Intron Marker Isna Arofatun Nikmah; Rodiyati Azrianingsih; Didik Wahyudi
Journal of Tropical Life Science Vol. 6 No. 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.05

Abstract

Porang (Amorphophallus muelleri Blume) dispersed and grows well in Java island. This research aimed to determine the genetic diversity of porang populations in Central Java and West Java based on LEAFY second intron (nuclear genome encoding flower development). DNA samples of porang were from previous study, which are obtained from Central Java (Pamedaran, Grobogan, Wonogiri, Karangtengah) and West Java (Cisompet) as ingroup. A. variabilis from Pamedaran Brebes, A. ochroleucus, A. longituberosus, A. sumawongii, and A. tinekeae from genbank were treated as outgroup. LEAFY second intron was amplified using primers FLint2 F1 5’-CTTCCACCTCTACGACCAGTG-3’ and FLint2 R1 5’-TCTTGGGCTTGTTGATGTAGC-3’. Analysis using dnasp5 showed that 23 samples had 89 invariable sites and 139 variable sites that consisted of 12 singleton two variants sites, 118 parsimony-informative two variants sites and 9 Parsimony-informative three variants sites. The total of haplotype were 7 haplotypes where 16 ingroup samples clumped on haplotype 1 whereas 2 samples of Grobogan (Grobogan I and Grobogan III) clumped on haplotype 2 and outgroup samples occupied at their own haplotype. Genetic distance of all ingroup samples were 0-0,004 showing no differences among individuals. Analysis of phylogenetic tree using Maximum Parsimony, Neighbor Joining and Maximum Likelihood resulting a polytomy branch of ingroup samples except Grobogan which forms a separated sub clad. Key words: Genetic diversity, haplotype, Java, LEAFY, Porang.
Effect of Nigella Sativa Extract on Inflammatory Cells, Interleukin-10, Interferon-γ and Histological of Kidney in Monosodium Glutamate-Induced Rats Abdalrauf A Mahmud Yousif; Hidayat Sujuti; Edi Widjajanto
Journal of Tropical Life Science Vol. 6 No. 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.04

Abstract

There is considerable evidence, suggest that, consumption of food additives monosodium glutamate (MSG), a flavor enhancer was unhealthy. Herbal medicine Nigella sativa (NS) has antioxidant properties able to cure the toxic induced by MSG. This study aimed to evaluate the risks of excessive use of MSG and to study the role of NS to inhibit inflammation and renal damage. Treated rats (twenty four male wistar rats) were divided into six group and analyzed by measuring the cells in blood, interleukin-10, interferon-γ serum levels by ELISA method and remove kidneys for histological examination. Histological of kidney for all groups except control, were showed different abnormalities include congestion of some blood vessels, hemorrhage  between tubules, widening in the renal tubules, revealed severe dilatation of Bowman's capsule and shrinkage of glomeruli, and areas of huge vacuole, were observed compared with control. Interleukin-10 was reduced in Groups 2,3,4 and 5, whereas increase in NS group compared with control. Interferon-γ was increased in groups 2,3,4 and reduced in groups 5,6 compared with control.  Eosinophil was increased in groups 2,5 and reduced in groups 3,4, 6 compared with control. This present study showed that administration of MSG to rats induced many changes effects on inflammatory cells, cytokines and histological of kidneys. NS has benefit in blood parameters, whereas harmful on kidney at these doses.
Genomic and Haplotype Comparison of Butanol Producing Bacteria Based on 16S rDNA Ekwan Nofa Wiratno; Suharjono Suharjono; Agustin Krisna Wardani
Journal of Tropical Life Science Vol. 6 No. 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.01

Abstract

High butanol demand for transportation was support to butanol development. Exploration of butanol producing bacteria using genome comparison and biogeography give role to butanol industrialization. Objective of this research are butanol production, genome comparison and haplotype analysis of butanol producing bacteria from Ranu Pani Lake sediment using 16S rDNA sequences. Highest butanol concentrations were resulted by Paenibacillus polymyxaRP 2.2 isolate (10.34 ± 0.00 g.l-1) then Bacillusmethylotrophicus RP 3.2 and Bacillusmethylotrophicus RP 7.2 isolate (10.11 ± 0.01 g.l-1 and 9.63 ± 0.01 g.l-1). Number of bases (T, C, A, G) of group 1 are similar, but different with group 2. Least G+C content is Clostridium saccharobutylicum Ox29 (51.35%) and highest is Bacillus methylotrophicus RP 7.2. Conserve region (1044 bp) of 16S rDNA higher then variative region (367 bp). The number of 319 bp is PIS whereas single tone as much as 48 bp. There are 17 conserves sequences. All of butanol producing bacterial sequences was clustered to 8 haplotype. Based on source of bacteria, there are three group of haplotype. Group A was isolated from Asia, group B was isolated from America and group C was isolated from Europe.
Effect of Methyl Parathion on Survival and Development of Tadpoles of Indian Cricket frog Fejervarya limnocharis Gurushankara H Puttaswamy Gowda; Sannanegunda Venkatarama Bhatta Krishnamurthy; Vasudev Venkateshaiah
Journal of Tropical Life Science Vol. 6 No. 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.08

Abstract

Amphibian populations are declining due to various causes including pesticide contamination in natural habitat. We evaluated effect of Methyl Parathion (MPT) an organophosphate pesticide on survival and development of common paddy field frog Fejervarya limnocharis in a laboratory condition. Effect of 0 µg MPT/L, 500 µg MPT/L, 1000 µg MPT/L, 1500 µg MPT/L, 2000 µg MPT/L and 3000 µg MPT/L was studied using static toxicity test for a duration of 28 days. MPT reduced the survival of tadpole. The mortality increased with the increased concentration of pesticide. The development decreased with increased MPT concentrations. At higher concentrations, MPT induced slow development and tadpoles failed to metamorphose. It is assumed that slow development could affect the larval life and amphibian population in agro-ecosystem.
Study on The Starch Granules Morphology of Local Varieties of Dioscorea hispida and Dioscorea alata Fauziah Fauziah; Shofiyatul Mas'udah; Hendrian Hendrian
Journal of Tropical Life Science Vol. 6 No. 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.09

Abstract

Starch is regarded as vital in the food industry, where granule size and shape determine its function and uses. One plant taxon that is widely known as a starch source is Dioscorea (Dioscoreaceae), whose starch and protein content make it a healthy food. As the initial step to identify which varieties possess potential as starch sources, we conducted study on starch granule morphology of some local varieties of D. hispida and D. alata. The aim of this study was to determine shape and size variation of starch granule of each variety of D. hispida and D. alata. The results showed that starch granule morphology does not only vary between species of D. hispida and D. alata, but also among varieties of those species. The shape of starch granules in D. hispida is dominantly polygonal (polyhedral). It showed similar results as those reported by other authors in their studies, except that – in this study - we found there is also a triangular shape, which has never been reported before. Starch granule size of D. hispida is found to be “Very small – Small” and only 1 variety of the species – that is gadung jahe – is classified as “Small” type. According to previous studies, varieties with small type starch granule are recommended as potential sources for non-food industrial raw materials. The shape of starch granules in D. alata is similar to those reported in other studies, which is dominantly triangular and polygonal; the size is classified as “Medium” type, except for two varieties that are uwi ratu and uwi ulo (DC5), which are classified as “Large”. According to previous studies, varieties with large type starch granule are recommended as potential sources for food. Thus, from this study 3 potential local varieties are identified, i.e. gadung jahe of D. hispida, and uwi ratu & uwi ulo of D. alata.
STS Marker Associated with Iron Toxicity Tolerance in Rice Lili Chrisnawati
Journal of Tropical Life Science Vol. 6 No. 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.11

Abstract

Iron (Fe) toxicity is one of the limiting factors for rice growth and production in paddy fields. The use of iron tolerant varieties is one of the most efficient way to solve this problems. Identification of molecular markers linked to the trait is very important to develop marker-assisted selection (MAS) to obtain tolerant lines. The objective of this study was to identify sequence tagged sites (STS) markers associated with iron tolerance trait in double haploid rice population. Forty-five double haploid lines derived from reciprocal double crossing, i.e. IR54/Parekaligolara//Bio110/ Markuti, were phenotypically screened in high Fe wetland rice field at Taman Bogo Experimental Station, Lampung. Molecular analysis performed using STS markers. The results of the association between the genetic and phenotypic analysis showed that there were three markers, i.e. OsIRT1, OsIRT2, and OsFRO2, associated with iron tolerance trait in rice. The markers have potential as selection markers for iron toxicity tolerant lines.

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